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下载福建十一选五:熒光修飾


多肽熒光修飾 - 染料和猝滅劑


AMC (7-Amino-4-methyl-coumarin)

UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

福建十一选五杀号技巧 www.xomtn.com AMC (7-Amino-4-methyl-coumarin

Cy3, Cy5

Cy3,Cy5是具有極高消光系數和熒光的染料。因此,它們特別適合于細胞中肽的非常靈敏的定位測定。它們的缺點是分子在福建十一选五杀号技巧條件下的高度不穩定性。因此,產率相對較低。

Cy3, Cy5

Dabcyl

Dabcyl is a non-fluorescent dye predominantly used as a quencher for other fluorophores (esp. Fluorescein type dyes, EDANS.). If Dabcyl is coupled to a peptide in close proximity to a fluorophore, it absorbs the emitted light of the fluorophore. Enlarging this distance (i.e. by enzymatic cleavage of the peptide) results in excitation of the fluorophore with an emission signal that can be detected.

Dabcyl

Dansyl

Dansyl is also used as a fluorophore quencher. Unlike Dabcyl, it inherits own fluorescence and thus might not be as useful for highly sensitive assays.

Dansyl

2,4-Dinitrophenyl (DNP)

2,4-Dinitropheny is a non-fluorescent dye that can be used as a fluorophore quencher (see Dabcyl for more details).

2,4-Dinitrophenyl (DNP)

DNP-Lysine

DNP-Lys是一種非熒光染料,可以用作熒光淬滅劑,用于多肽定制(參考Dabcyl)

DNP-Lysine

EDANS (5-((2-aminoethyl)amino)napthalene-1-sulfonic acid)

EDANS is a commonly used dye in FRET (fluorescence resonance energy transfer) peptides in combination with Dabcyl as quencher.

EDANS (5-((2-aminoethyl)amino)napthalene-1-sulfonic acid)

Fluorescein

Fluorescein is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

Fluorescein

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD is a fluorescent dye, used for amine modification.

NBD (7-nitrobenz-2-oxa-1, 3-diazole)n

p-Nitro-Aniline

p-Nitro-Aniline is a chromogen used as colorimetric enzyme substrate in many standard enzyme assays in cuvettes.

p-Nitro-Anilinen

Rhodamine B

Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescent peptide assays.

Rhodamine B

Tamra

Tamra is the most commonly used rhodamine dye in fluorescence assays.

Tamra